Chemidoc MP / Bio-Rad, upgrade kit for fluorescence

Gel imagers are essential devices in every molecular biology and medical laboratory and enable genetic material and protein samples to be analysed qualitatively and quantitatively. This can provide crucial insights into the molecular biological processes within a cell and is used worldwide as a robust method for detecting protein expression and analysing genetic traits. The research location Krems is home to a large number of research groups with a molecular biology focus that benefit from such a device.

Location: Karl Landsteiner University of Health Sciences
Contact person: Prof. Dr. Gerald Obermair and Prof. Dr. Dagmar Stoiber-Sakaguchi

Optima MAX BioSafe Ultracentrifuge / Beckman Coulter

Ultracentrifuges are devices that generate gravitational fields through high rotational speeds and can thus cause solid components of a solution to sediment. This can be used to pelletise cells, nucleic acids or other solid components, but also to create density gradients. Ultracentrifuges are widely used in sample preparation and are essential for molecular biology laboratories.

Location: Karl Landsteiner University of Health Sciences
Contact person: Prof. Dr. Gerald Obermair and Prof. Dr. Dagmar Stoiber-Sakaguchi

qPCR Light Cycler 96 / Roche

Real-time PCR (RT-PCR) devices enable the amplification and detection of very small amounts of DNA and can be used to quantify gene expression patterns, among other things. RT-PCR is a globally established and recognised method and corresponding devices are part of the standard repertoire of every molecular biology and genetic research facility.

Location: Karl Landsteiner University of Health Sciences
Contact person: Prof. Dr. Gerald Obermair and Prof. Dr. Dagmar Stoiber-Sakaguchi

Ultracentrifuge CP 80NX

The Ultracentrifuge CP 80NX from Eppendorf Himac Technologies is equipped with a titanium P40ST  swinging bucket rotor that can achieve a maximum relative centrifugal force (RCF) of 284,000 x g (40.000 rpm). Ultracentrifugation is employed to separate cellular organelles, to purify proteins or viral particles, and to isolate nucleic acids based on their density, size, and molecular weight. The rotor is ideal for density gradient centrifugation, which is typically applied for the isolation of extracellular vesicles. The rotor can be operated with 4 mL or 13 mL tubes and a maximum volume of 6 x 13 mL can be centrifuged per run.

Location: University for Continuing Education Krems, D3.09
Contact person: Susanne Krenn and Vladislav Semak, PhD

Agilent Bioanalyzer 2100

The Agilent 2100 Bioanalyzer system offers fast and reliable separation, sizing and quantification of proteins, DNA, and RNA by on-chip electrophoresis. The system enables high-sensitive detection in the picogram range for DNA, RNA, and protein analysis with minimal sample consumption (1 µL for nucleic acids and 4 µL for diluted proteins). Results are obtained in less than 30 minutes.

Material required:

• Agilent DNA kits (ideal for automated sizing and quantification of PCR fragments, restriction digests or fragmented DNA)
• Agilent RNA kits (for fast, easy and precise integrity checks and sample quantification)
• Agilent protein kits (for the assessment of protein concentration, identity, and purity in a wide variety of samples)

Location: University for Continuing Education Krems, D3.23
Contact person: Sabrina Summer, PhD

Parallelfermentersystem:
DG-System; Configurable DASGIP System

The DASGIP multi-fermenter system is an advanced bioprocessing platform designed for the cultivation of microbial and cell cultures. It allows for the simultaneous operation of multiple bioreactors, enabling high-throughput experimentation and optimization of bioproduction processes. The system is equipped with sophisticated control features for monitoring and adjusting crucial parameters such as temperature, pH, dissolved oxygen levels, and agitation speed. This level of control ensures precise and reproducible culture conditions, leading to consistent results and improved scalability. Additionally, the DASGIP multi-fermenter system offers flexibility in vessel configurations, accommodating various vessel sizes and types to suit different applications and experimental setups. Overall, it is a versatile and efficient tool for bioprocess development and optimization in research and industrial settings.

Technical details:

76 SR1000ODLS: Vessel size: 4 x 400 mL, Rushton Turbine: 2x with 4 blades

76DGCOND30: Offgas-condenser

76DGPHHMC220: pH-Sensor

76DGPOHVF220: DO-sensor

76DGLVL220: Antifoam-/Level-sensor

76DGTC4SC4B: Temperature control up to 99°C, Gas-mix module with up to 4 gases

76DGMX44H: Gasflow controlled by massflow-controler DASGIP MX4/4

76DGMP8: 16 flow controlled pumps

76DGIWF: Inline water filtration system

Location: IMC University of Applied Sciences Krems
Contact person: Prof.(FH) DI Dominik Schild 

IXplore SPIN ScanR NoviSight
(confocal high content imaging microscope)

The microscope enables optimal imaging and precise 3D high content analysis of physiologically relevant 3D models such as reconstructed tumours, full skin equivalents or other 3D tissues.

The following applications are possible:

3D high-content analysis
Real object counting and morphological measurements in 3D.
Statistical analysis of microplate data (96 & 384 well plates).
Detection of rare cellular events.
Life Cell Imaging: Investigation of living cells using time-lapse microscopy.

Technical Details

Inverted motorised microscope stand for incident and transmitted light observation with 2 decks for optical module mounts and 1 slot for optional Z-drift compensator. Light intensity, observation, objective position, mirror turret position, beam path, fluorescence shutter position are shown on the integrated display. Beam path options for binocular tube and left side exit: 0:100, 50:50, 100:0. Motorised coaxial coarse and fine drive with nosepiece for raising/lowering, focusing stroke 9.5 mm. Maximum focussing speed: 3 mm/sec. Minimum step size: 0.01 μm. Optional accessory U-MCZ-1-2 for manual Z control. Compatible with transmitted light illumination IX3-ILL, IX3-RFA, IX3-RFAL, IX3-RFALFE reflected light illumination, IX3-SSU, BX3-SSU-1-2, IX3-SVR, IX3-SVL, IX2-SP, IX2-GS, GX-SVR, GX-SFR stages. Requires IX3-CBH control unit. Includes 6-position motorised IX3-D6REA nosepiece, touch panel control, jigs, power supply, cable clamps, control panel and nameplate.

Light source: IX3-LHLEDC-1-3 LED

White true colour LED light source for the IX83P2ZF, IX83P1ZF, IX73P2F, IX73P1F microscope stands with an intensity comparable to 30 W halogen light. Coupling to the microscope via the IX3-ILL transmission column. Light control via touch panel controller of IX83, U-MCZ or front control panel of IX73. Average LED service life 20,000 hours. U-LEDPS is required for use with IX73.

Laser:

• SD-COMB Laser Combiner
• IX-LAS405-50LXS laser 405 nm
• IX-LAS488-100LSS 488 nm laser
• IX-LAS561-100LSS laser system (561 nm)
• IX-LAS640-100LXS laser 640nm

Objectives:

Universal planapochromat objectives:

• UPLXAPO4X Objective (4X)
• UPLXAPO10X Objective (10X)
• UPLXAPO20X Objective (20X)

Universal apochromat water immersion objective:

• UAPON20XW340 NA 0.7 AA 0.35*BTS (20X)
• UAPON40XW340 NA1.15AA 0.25*BTS (40X)
• UPLSAPO60XW/1.2 objective (60X)

Universal Plan Semi-Apochromat, phase contrast objective:

• UCPLFLN20XPH (20X)

Filter: CSUW1P-DE09 Em. Filter 488/561
DAPI, FITC, Cy3, TexasRed, Cy5, Cy7

Software Modules:

• CS-S-DL-VF Deep Learning TruAI Solution
• CS-DI-V4.1 Dimension
• CS-DI-DT-V4.1 Dimension DT
• NS-ASW NoviSight main licence

Location: IMC University of Applied Sciences Krems / D2.05
Contact person: Prof.(FH) Mag. Dr. Christoph Wiesner

HP Workstation Z8 G4, Schrödinger Software

Advanced molecular modeling and 3D visualization of biomolecules

Location: IMC University of Applied Sciences Krems / D2.07
Contact person: Prof.  (FH) Dr. Christian Klein

Stereo-Monitor 3D PluraView Impact 24″ 

Full HD 144Hz B

Location: IMC University of Applied Sciences Krems / D2.07
Contact person: Prof.  (FH) Dr. Christian Klein

MinON next generation sequencing device, Oxford Nanopore

The Oxford Nanopore next generation sequencing device uses flow cells which contain nanopores embedded in an electro-resistant membrane. Each nanopore corresponds to its own electrode connected to a channel and sensor chip, which measures the electric current that flows through the nanopore. When a molecule passes through a nanopore, the current is disrupted to produce a characteristic ‘squiggle’. The squiggle is then decoded using basecalling algorithms to determine the DNA or RNA sequence in real time.

The device allows:

• Direct DNA/RNA sequencing; scalable
• Sequencing in real-time for rapid access to time critical information (e.g. pathogen identification), the generation of early sample insights and more control over the sequencing experiment
• Call base modifications simultaneously with nucleotide sequence
• Long reads to improve assembly and characterization of repetitive and difficult regions
• Applications include whole genome sequencing, targeted sequencing, metagenomics, epigenetics, epitranscriptomics.

Location: IMC University of Applied Sciences Krems /  D.E.01
Contact person: Prof. (FH) Priv.-Doz. Dr. Reinhard Klein